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Determination of Ubidecarenone (Coenzyme Q10, Ubiquinol-10) in Raw Materials and Dietary Supplements by High-Performance Liquid Chromatography with Ultraviolet Detection: Single-Laboratory Validation

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Abstract:

A method based on high-performance liquid chromatography with ultraviolet detection has been developed to quantify ubidecarenone [coenzyme Q10 (CoQ10)] in raw materials and dietary supplements. Single-laboratory validation has been performed on the method to determine repeatability, accuracy, selectivity, limits of detection and quantification (LOQ), ruggedness, and linearity for CoQ10. As CoQ10 can exist as the biologically active reduced form, the application of an oxidizing agent, ferric chloride, drives the equilibrium mechanics to the fully oxidized state and allows for exact quantification of total CoQ10 in the sample. This method was found to be fit and linear for the testing of materials containing CoQ10 in the range of 501000 mg/g. Repeatability precision for CoQ10 was between 2.15 and 5.00 relative standard deviation. Observed recovery of CoQ10 was found to be between 93.8 and 100.9. LOQ was found to be 9 g/mL. Further, limited studies showed that some adulterants and degraded material could be satisfactorily separated from CoQ10 and identified.

Document Type: Research Article

Affiliations: 1: Eurofins Scientific, 1365 Redwood Way, Petaluma, CA 94954. 2: Soft Gel Technologies Inc., 6982 Bandini Blvd, Los Angeles, CA 90040. 3: Compound Solutions, 2215 Vineyard Ave, Escondido, CA 92029. 4: Pharmavite LLC, 1150 Aviation Place, San Fernando, CA 91340. 5: Tampa Bay Analytical Research, PO Box 931, Safety Harbor, FL 34695.

Publication date: September 1, 2007

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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