A high-performance liquid chromatographic method for determining apramycin in animal feeds was developed. Apramycin in feeds was extracted with 0.1 M HCl solution and cleaned up with an MCX solid-phase extraction column. The purified extract was derivatized with o-phthaldehyde,
and components were separated on a C18 column and detected with a fluorescence detector. Mass spectrometric data confirmed that apramycin was derivatized at all the 4 primary amines on the apramycin molecule. Average recoveries at 8 included levels (5, 10, 20, 40, 80, 200, 400, and 2000 mg/kg)
ranged from 92.2 to 100.5%, and the coefficients of variation were <6.5%. Standard curves were linear over the range 0.05 to 10 g/mL. The detection and quantitation limits were determined to be 0.2 and 1.0 mg/kg, respectively.
Document Type: Research Article
China Agricultural University, State Key Laboratory on Animal Nutrition, Beijing 100094, People's Republic of China.
Publication date: July 1, 2007
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