Multiresidue Determination of Zeranol and Related Compounds in Bovine Muscle by Gas Chromatography/Mass Spectrometry with Immunoaffinity Cleanup
Abstract:A gas chromatography/mass spectrometry (GC/MS) method with immunoaffinity cleanup was developed for the determination of zeranol and related compounds, taleranol, zearalanone, and -zearalenol in bovine muscle. Muscle samples were extracted with methanol and cleaned up with immunoaffinity chromatography (IAC) columns containing monoclonal antibodies raised against zeranol coupled to CNBr-activated Sepharose 4B. After derivatization, the compounds were analyzed by GC/MS. The dynamic column capacities for zeranol, taleranol, zearalanone, and -zearalenol were 2639.7, 2840.3, 2731.5, and 2736.3 ng/mL Sepharose gel, respectively. The limits of detection and quantification were 0.5 and 1.0 ng/g, respectively, for all 4 compounds. Mean recoveries were 79.6110.7% with coefficients of variation of 3.211.4% at spiked levels of 1.05.0 ng/g. This IAC-GC/MS method may be used for the determination of zeranol, taleranol, zearalanone, and -zearalenol residues in bovine muscle, and possibly other tissues.
Document Type: Research Article
Affiliations: China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, People's Republic of China.
Publication date: November 1, 2006
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