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A Novel Correlation for Rapid Lactose Determination in Milk by a Cryoscopic Technique

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Residual lactose in special milk was systematically determined for people with lactose intolerance by means of a rapid on-line measurement of the cryoscopic point. A proposed cryoscopic procedure was compared to 2 conventional yet highly laborious methods: the enzymatic procedure with spectrophotometric control and the polarimetric method. Several experiments with different mixtures of both semi-skimmed and low-lactose milk were performed. A lineal relationship was found between lactose concentration and freezing point, the analytical equation for which shows a close relationship regarding the 3 methods used. The advantages of the cryoscopic procedure include speed in obtaining results and operational simplicity at a low cost, better monitoring of enzymatic hydrolysis kinetics, and greater control over the production process for delactosed milk. The equation obtained also enables prediction of the lactose percentage in commercial milk by a simple measurement of freezing point.
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Document Type: Research Article

Affiliations: 1: Universidad de Valladolid, Dpto. Ingeniera Agrcola y Forestal (rea de Tecnologa de los Alimentos), E.T.S. Ingeniaras Agrarias, Avda. Madrid 44, 3407 Palencia, Spain. 2: Universidad de Valladolid, Dpto. Ingeniera Agrcola y Forestal (rea de Tecnologa de los Alimentos), E.T.S. Ingeniaras Agrarias, Avda. Madrid 44, 3407 Palencia, Spain: Present address: Central Lechera Vallisoletana LAUKI, Avda. Santander, 2. 47010 Valladolid, Spain.

Publication date: 2006-11-01

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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