UV Spectrophotometric Determination of Bioflavonoids from a Semisolid Pharmaceutical Dosage Form Containing Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham Standardized Extract: Analytical Method Validation and Statistical Procedures
Abstract:A precise, accurate, and sensitive UV spectrophotometric method was developed and validated for routine quantification of total bioflavonoids, expressed as rutin, from a topical oil-in-water pharmaceutical emulsion containing the extract of Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham. The method was validated experimentally, and the data were treated rigorously by statistical analysis. The following analytical parameters were assessed: linearity, specificity, intra- and interrun precision measured as relative standard deviation (RSD, %), intra- and interrun accuracy (E, %), recovery (Rec., %), limit of detection (LOD, g/mL), and limit of quantification (LOQ, g/mL). The UV spectrophotometric method was linear (r 2 0.9995) for standard rutin over the concentration range of 5.015.0 g/mL with specificity for total bioflavonoids (expressed as rutin) at 361.0 nm with an absence of interferents from the complex matrix; RSD of 1.79%, intrarun (E 97.88 1.75 to 99.0 0.33%) and interrun (E 98.38 1.12 to 100.79 1.30%) accuracy; Rec. 98.64 0.42 to 100.74 0.41%; LOD 0.20 g/mL; and LOQ 0.30 g/mL.
Document Type: Research Article
Affiliations: 1: University of So Paulo, School of Pharmaceutical Sciences, Department of Pharmacy, FCF-USP, 580 Prof. Lineu Prestes Ave, Bloco 13, Conjunto das Qumicas, Cidade Universitria, 05508-900, So Paulo, Brazil. andrerbusp.br; andre_rolimuol.com.br 2: University of So Paulo, School of Pharmaceutical Sciences, Department of Pharmacy, FCF-USP, 580 Prof. Lineu Prestes Ave, Bloco 13, Conjunto das Qumicas, Cidade Universitria, 05508-900, So Paulo, Brazil.
Publication date: November 1, 2006
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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