A Validated Liquid Chromatographic Method for Estimation of Solasodine in Various Solanum Species and Market Formulations
Abstract:This paper describes an improved liquid chromatographic (LC) method involving a prechromatographic derivatization step for the estimation of solasodine from berries of various Solanum species, market samples of Solanum xanthocarpum herb, extract, and its market formulations. Solasodine has heterocyclic nitrogen but has no conjugated double bonds in its structure. However, in all reported LC methods, detection was made in the ultraviolet range of 200213 nm. In the present study, a prechromatographic derivatization of solasodine was done by forming an ion-pair complex of the heterocyclic nitrogen using the acidic dye methyl orange and acetate buffer of pH 4.7. Detection could be made in the visible range at 530 nm in this method. The method was validated and successfully applied to determine solasodine content in various plant samples and polyherbal formulations. The relative standard deviation was found to be 0.025% for system precision, and 0.8% for the linearity of the method, and the correlation coefficient was 0.999. Plant samples and market formulations were analyzed and found to contain solasodine in the range of 0.1130.227% (w/w) on a fresh weight basis in berries; 0.31.278% (w/w) and 0.412% (w/w) on a dry weight basis in S. xanthocarpum herb powder and extract, respectively; and 0.2450.525% (w/w) on dry weight basis in formulations containing S. xanthocarpum herb powder. No matrix interference was encountered. The method was found to be accurate, with a mean recovery of 100.5 0.83%. The method has good reproducibility and was found to be suitable for estimation of solasodine.
Document Type: Research Article
Affiliations: 1: K.B. Institute of Pharmaceutical Education and Research, Sector-23, GH-6, Gandhinagar PIN Code 382023, Gujarat, India. 2: Synchron Research Pvt. Ltd., The Chambers, Sarkhej Gandhinagar Highway, Ahmedabad, PIN Code 380 054, Gujarat, India. 3: L.J. Institute of Pharmacy, Near Nagdev Kalyan Mandir, Sanand Cross Rds, Ahmedabad, PIN Code 382 210, Gujarat, India.
Publication date: November 1, 2006
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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