Determination of Lomefloxacin in Tablet Preparations by Liquid Chromatography
Authors: Tozo, Greci C.G; Salgado, Hrida R.N
Source: Journal of AOAC International, Volume 89, Number 5, September 2006 , pp. 1305-1308(4)
Publisher: AOAC International
Abstract:A sensitive, precise, and specific high-performance liquid chromatography (HPLC) method was developed for the assay of lomefloxacin (LFLX) in raw material and tablet preparations. The method validation parameters yielded good results and included the range, linearity, precision, accuracy, specificity, and recovery. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay. The HPLC separation was performed on a reversed-phase Phenomenex C18 column (150 4.6 mm id, 5 m particle size) with a mobile phase composed of 1% acetic acidacetonitrilemethanol (70 + 15 + 15, v/v/v), pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 280 nm. The calibration graph for LFLX was linear from 2.0 to 7.0 mg/mL. The interday and intraday precisions (relative standard deviation) were less than 1.0%. The method was applied for the quality control of commercial LFLX tablets to quantitate the drug.
Document Type: Research Article
Affiliations: Faculdade de Cincias Farmacuticas, Programa de Ps-Graduao em Cincias Farmacuticas, UNESP, Rod. Araraquara-Ja, km 1, CEP 14801-902, Araraquara, SP, Brazil.
Publication date: September 1, 2006
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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