Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Methods 996.09, VIP^® for EHEC and 996.10, Assurance EIA^® EHEC with the Reference Culture Method for the Detection of Escherichia coli O157:H7 in Beef

Authors: Feldsine, Philip T.; Green, Shannon T.; Lienau, Andrew H.; Kerr, David E.

Source: Journal of AOAC International, Volume 88, Number 4, July 2005 , pp. 1193-1196(4)

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Abstract:

The Visual Immunoprecipitate Assay (VIP^®) method for the detection of enterohemorrhagic Escherichia coli O157:H7 (VIP for EHEC) and Assurance^® Enzyme Immunoassay (EIA) method for the detection of EHEC (EHEC EIA) are AOAC INTERNATIONAL Official Methods 996.09 and 996.10, respectively. A minor modification to the enrichment medium used in both methods has been developed. This modification, the BioControl modified EHEC medium™ (BioControl mEHEC™) provides a more cost-effective procedure with performance equivalent to that of the cultural method for detection of E. coli O157:H7 in beef.

Document Type: Research article

Affiliations: 1: BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005.

Publication date: 2005-07-01

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The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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