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A Simple Determination of Mizoribine in Human Plasma by Liquid Chromatography with UV Detection

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Abstract:

A simple liquid chromatography (LC) method was developed for determination of the therapeutic level of mizoribine in human plasma. After precipitation of plasma proteins with 6% perchloric acid, mizoribine was determined by LC with spectophotometric detection. The peak height for mizoribine was linearly related to its concentrations, which ranged from 0.09 to 3.13 μg/mL. Therefore, the limit of quantitation was considered to be 0.09 μg/mL. The accuracy was 104.96–107.37%. The intra- and interday relative standard deviation values were in the range of 1.10–3.25%. The detection limit was 0.025 μ g/mL, defined as a signal-to-noise ratio of 3. The plasma concentrations of mioribine were not related to the dosage. Because mizoribine was mainly excreted in the urine, the plasma concentrations of mizoribine might be affected by a change in renal function. Therefore, the mizoribine concentration in blood should be monitored and the dosage adjusted, depending on the condition of renal function. It was suggested that the present method may be applied well in the therapeutic drug monitoring for mizoribine.

Document Type: Research Article

Affiliations: 1: Shujitsu University, Department of Clinical Pharmacy, School of Pharmacy, Okayama, Japan 703-8516. 2: Fukuoka University, Faculty of Pharmaceutical Sciences, Fukuoka, Japan 814-0180.

Publication date: July 1, 2005

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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