Determination of Residual Clopidol in Chicken Muscle by Capillary Gas Chromatography/Mass Spectrometry
Clopidol was extracted from chicken with acetonitrile, and the extract was evaporated to about 5 mL. The concentrated solution was centrifuged and applied to a glass column filled with basic alumina. The column was eluted with methanol and the methanol was evaporated to dryness. The residue was derivatized with Sylon BFT™ and analyzed by gas chromatography/mass spectrometry (GC/MS). Mass characteristics of trimethylsilyl derivative of clopidol were interpreted manually. The selected-ion monitoring mode was performed at m/z 191, 248, 263, and 265. A sensitive, reproducible GC/MS method was developed for monitoring residues of clopidol in chickens. All recoveries of the drug from chicken muscle fortified at 1, 10, and 100 ng/g were >65%, and relative standard deviations were <15%. The detection limit was about 0.5 ng/g, and the quantitation limit was the lower limit of the standard curve, which was about 1 ng/g.
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Document Type: Research Article
Affiliations: South China Agricultural University, Centre for Veterinary Drug Residues, College of Veterinary Medicine, Guangzhou, Guangdong, PC 510642, People's Republic of China.
Publication date: 01 July 2005
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