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Validation Assay for Total Flavonoids, as Rutin Equivalents, from Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) Commercial Extract

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An ultraviolet spectrophotometric method was validated for total flavonoid quantitation, as rutin equivalents, present in the Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) commercial extract. Parameters as linearity, interval (range), specificity, estimated limit of detection (LOD, μg/mL), estimated limit of quantitation (LOQ, μg/mL), recovery (R, %), precision or relative standard deviation (RSD, %), and accuracy (E, %) were established. The analytical method was validated according to the experimental results: correlation coefficient (r = 0.9997); interval (RSD = 0.15–0.47%; E = 98.98–101.24%); specificity to total flavonoids quantitation, as rutin equivalents, at wavelength 361.0 nm; LOD = 0.09 μg/mL and LOQ = 0.27 μg/mL; R = 99.36–102.14%; adequate intraand interrun precision (0.30–0.49% and 0.31–0.81%), and intra- and interrun accuracy (100.60–102.38% and 98.58–100.38%).

Document Type: Research Article

Affiliations: University of São Paulo, Department of Pharmacy, Faculty of Pharmaceutical Sciences, 580 Prof. Lineu Prestes Ave, Bl. 13, Conjunto das Químicas, 05508-900, São Paulo, Brazil.

Publication date: 2005-07-01

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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