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Fast Antimicrobial Screen Test (FAST): Improved Screen Test for Detecting Antimicrobial Residues in Meat Tissue

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Abstract:

The Fast Antimicrobial Screen Test (FAST) is a simple and quick screening test developed to detect antibiotic and sulfonamide residues in food animal carcasses in slaughter establishments. This microbial inhibition test detects antimicrobials that are allowed to be used in food animals. It has the ability to detect these antimicrobials at or above the allowable limit in carcass kidney fluids in 6 h. Laboratory evaluations show that the lower limit of detection (LLD) of FAST and the Calf Antibiotic and Sulfa Test (CAST) for antibiotics tested was the same, but the LLD for sulfonamides of FAST was lower than the LLD of CAST. Compared with the Swab Test on Premises (STOP) developed in 1977, the LLD's of FAST for both antibiotics and sulfonamides were significantly better. Under field conditions, the sensitivity of FAST and CAST to antibiotic and sulfonamide residues in animal kidneys was not significantly different, but the time required by FAST was significantly lower than CAST (6 versus 18 h). Compared with the STOP, the sensitivity and the range of detection by FAST for all antimicrobials were significantly higher and the testing time was lower (18 versus 6 h).

Document Type: Research Article

Affiliations: 1: U.S. Department of Agriculture, Food Safety and Inspection Service, Methods Development Laboratory, Beltsville, MD 20705; Present address: U.S. Department of Agriculture, Food Safety and Inspection Service, Zoonotic Disease and Residue Surveillance Division, Washington, DC 20250; Present name and location: Laboratory QA/QC Division, USDA, FSIS, OPHS, Russell Research Center, Suite 205, 950 College Station Rd, PO Box 6085, Athens, GA 30604. 2: U.S. Department of Agriculture, Food Safety and Inspection Service, Methods Development Laboratory, Beltsville, MD 20705; Present address: U.S. Department of Agriculture, Food Safety and Inspection Service, Field Automation and Information Management Division, Washington, DC 20250; Present name and location: Laboratory QA/QC Division, USDA, FSIS, OPHS, Russell Research Center, Suite 205, 950 College Station Rd, PO Box 6085, Athens, GA 30604. 3: U.S. Department of Agriculture, Food Safety and Inspection Service, Methods Development Laboratory, Beltsville, MD 20705; Present address: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Riverdale, MD 20740; Present name and location: Laboratory QA/QC Division, USDA, FSIS, OPHS, Russell Research Center, Suite 205, 950 College Station Rd, PO Box 6085, Athens, GA 30604.

Publication date: March 1, 2005

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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