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Determination of Chlorpheniramine Maleate and Tincture Ipecac in Dosage Form by Liquid Chromatography with Ultraviolet Detection

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A procedure was developed and validated for measuring chlorpheniramine maleate and tincture ipecac (as emetine hydrochloride) by reversed-phase liquid chromatography with methanol–10mM sodium heptanesulfonate (20 + 30) as the mobile phase; the pH was adjusted to 4 with acetic acid, and the flow rate was at 1.5 mL/min, with ultraviolet detection at 254 nm. Propyl paraben was used as the internal standard. The standard curves were linear (r = 0.998 and 0.9998) for both chlorpheniramine maleate and emetine hydrochlo-ride over the ranges of 5–100 and 0.1–40 μg/mL, respectively. The mean recoveries ± standard deviation were 101.37 ± 2.77% for chlorpheniramine maleate and 98.8 ± 1.47% for emetine hydrochlo-ride. The proposed method was applied to the determination of chlorpheniramine maleate alone in tablet and syrup dosage forms. The method also was applied to the determination of the emetine content of ipecac liquid extract and tincture ipecac; the results were compared with those of the method of the British Pharmacopoeia. The proposed method was applied successfully to the simultaneous determination of chlorpheniramine maleate and tincture ipecac, as emetine hydrochlo-ride, in syrup dosage form. Both drugs and the internal standard were separated from all interfering components in <5 min. The proposed method is simple, specific, and economical, when compared with other published methods that determine each component alone.

Document Type: Research Article

Affiliations: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Tanta, Egypt

Publication date: July 1, 2003

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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