Comparison Between a Bioassay and Liquid Chromatography– Fluorescence–Mass Spectrometryn for the Determination of Incurred Enrofloxacin in Whole Eggs
Abstract:The objective of this study was to compare a bioassay with a liquid chromatography–fluorescence–mass spectrometryn method for detection of enrofloxacin (ENRO) in incurred eggs. The bioassay developed by our laboratories involves an agar diffusion microbiological method using Klebsiella pneumoniae as an indicator organism. Results demonstrate that both methods are capable of detecting incurred fluoroquinolone residues in eggs. During the 3-day dosing period of hens (Days 1–3) and following drug withdrawal (Days 5, 7, and 9), both of these methods were able to detect incurred ENRO in eggs above the zero tolerance established by the U.S. Food and Drug Administration. The LC–fluorescence–MSn method has the benefit of providing confirmation for fluoroquinolones, while the bioassay may be used as an effective, rapid screening method for detection of fluoroquinolone residues in eggs.
Document Type: Research Article
Affiliations: 1: University of Arkansas, Department of Poultry Science, Fayetteville, AR 72701 2: U.S. Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, 600 E. Mermaid Ln, Wyndmoor, PA 19038
Publication date: July 1, 2003
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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