Interference-Free Biosensor Based on Screen-Printing Technology and Sol-Gel Immobilization for Determination of Acetaldehyde in Wine
Abstract:A monoenzymatic amperometric biosensor was developed for the detection of acetaldehyde. The sensor is based on the association of screen-printed carbon electrodes and aldehyde dehydrogenase immobilized by a sol-gel entrapment method. Modification of screen-printed carbon electrodes with Reinecke salt of Meldola's Blue (MBRS) resulted in highly sensitive and interference- free nicotinamide-adenine dinucleotide (NADH) detectors. Based on MBRS-mediated oxidation of NADH at –150 mV versus pseudo Ag/AgCl, acetaldehyde was determined in the range 10–260 μM, compatible with wine quality monitoring. The method of immobilization based on sol-gel entrapment was optimized to obtain the best compromise between sensitivity and operational stability. The sensor response was stable for 40 consecutive assays with methyltrimethoxysilane used as alkoxide precursor, thus allowing a possible calibration of the sensor before each measurement. The biosensors were used to analyze French wines. The method was validated with a commercially available enzymatic kit based on a standard spectrophotometric method.
Document Type: Research Article
Affiliations: 1: Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France. 2: University of Bucharest, Department of Analytical Chemistry, 90-92 Panduri, Sect. 5, Bucharest 76234, Romania. 3: University of Bucharest, Department of Analytical Chemistry, 90-92 Panduri, Sect. 5, Bucharest 76234, Romania; Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France.
Publication date: 2002-11-01
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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