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Use of Hydrophilic Hydroxypropyl Methacrylate/Ethylene Glycol Methacrylate Packing Material in Size-Exclusion Chromatography

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Abstract:

Spherical particles of hydroxypropyl methacrylate/ethylene glycol methacrylate copolymer were synthesized in-house for use in size-exclusion chromatography. The porous hydrophilic material was packed in glass and stainless steel columns to evaluate their chromatographic performance. The support particles were small (approximately 20 Å), and the average pore size was in the low range of mesopores (approximately 100 Å). The packed columns were calibrated by using polysaccharide dextrans, showing a good range of separation for molecular weights between 10 000 and 600 000 daltons. The packing material appears to separate the large molecules through the size-exclusion mechanism. Polysaccharides and polypeptides dissolved in adequate mobile phases were injected into the packed column. The separation of the macromolecules was consistent with the size-exclusion mechanism. Application of the packing material to the separation of small molecules (alkyl alcohols) was also investigated.

Document Type: Research Article

Affiliations: 1: Temple University School of Pharmacy, Department of Pharmaceutical Sciences, 3307 N. Broad St, Philadelphia, PA 19140; Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, UFMG, Av. Olegário Maciel, 2360, Belo Horizonte, MG, 30180-112, Brazil. 2: Temple University School of Pharmacy, Department of Pharmaceutical Sciences, 3307 N. Broad St, Philadelphia, PA 19140.

Publication date: November 1, 2002

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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