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Determination of Polycyclic Aromatic Hydrocarbons from Buzzards (Buteo buteo) and Tawny Owl (Strix aluco) by Liquid Chromatography with Fluorescence Detection

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Supercritical fluid extraction was applied to the determination of naturally contaminated polycyclic aromatic hydrocarbons (PAHs) in bird tissue by liquid chromatography with fluorescence detection (LC-FL). Recoveries (>90%) and relative standard deviations (≤7.7%) were satisfactory. The levels of 10 PAHs were analyzed in 6 classes of tissues (heart, liver, intestine, muscle, lung, and kidney) of 10 buzzards and 2 tawny owls, predatory birds from the Galicia (northwest Spain). The PAHs found most abundantly were pyrene, fluoranthene, benzo[a]anthracene, and anthracene. Chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, and indeno[1,2,3-cd]pyrene were not detected. Intestine, kidney, and lung were more polluted than other tissues.

Document Type: Research Article

Affiliations: 1: University of Santiago de Compostela, Faculty of Pharmacy, Department of Analytical Chemistry, Nutrition and Bromatology, Area of Nutrition and Bromatology, E-15782 Santiago de Compostela, Spain. 2: University of Santiago de Compostela, Faculty of Pharmacy, Department of Analytical Chemistry, Nutrition and Bromatology; and University of Santiago de Compostela, Faculty of Pharmacy, Institute of Research and Food Analysis, Laboratory of Bromatology, E-15782 Santiago de Compostela, Spain.

Publication date: January 1, 2002

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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