Optimization and Validation of Arsenic Determination in Foods by Hydride Generation Flame Atomic Absorption Spectrometry
Abstract:A hydride generation flame atomic absorption spectrometric method was developed and optimized to quantitate arsenic (As) in foods. A wet digestion of the samples with HNO3 + H2O2 was performed and excess oxidants were eliminated by addition of hydrochloric acid and urea. As5+ in As3+ was then reduced by potassium iodide. The As3+ solution was analyzed by generation of arsine with sodium tetrahydroborate. As determination ranged from 2.5 to 20 μg/L, with a determination coefficient of 0.997. The limits of detection (LOD) and quantitation (LOQ) were 0.6 and 2.1 μg/L, respectively. The method was validated and good results were obtained for recovery, precision, accuracy, LOD, and LOQ. This method is now used to analyze foods from Rwanda.
Document Type: Research Article
Affiliations: 1: Université Nationale du Rwanda, Butare, Rwanda; and Toxicologie et Bromatologie, Université de Liège, Domaine Universitaire du Sart-Tilman, B-23, 4000 Liège, Belgium. 2: Toxicologie et Bromatologie, Université de Liège, Domaine Universitaire du Sart-Tilman, B-23, 4000 Liège, Belgium. 3: Analyse des Médicaments, Université de Liège, Domaine Universitaire du Sart-Tilman, B-36, 4000 Liège, Belgium. 4: Chimie Analytique, Université de Liège, Domaine Universitaire du Sart-Tilman, B-36, 4000 Liège, Belgium.
Publication date: January 1, 2002
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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