Size Exclusion Chromatographic Cleanup for GC/MS Determination of Organophosphorus Pesticide Residues in Household and Vehicle Dust
Size exclusion chromatography (SEC) was used as a cleanup method for the analysis of organophosphorus pesticides in household and vehicle dusts. The pesticides investigated were diazinon, methyl parathion, chlorpyrifos, malathion, phosmet, and azinphosmethyl. These compounds are of
interest due to their use in agricultural tree fruit production and/or urban pest control. Pesticides were determined via gas chromatography/mass spectrometry with selected-ion monitoring and cool on-column injection. The lower limit of method validation was 0.20 μg/g. Method limits of
detection in dust ranged from 0.012–0.055 μg/g. Dust samples were collected with vacuums from the homes and vehicles of people living and working in a rural agricultural region in the central part of Washington State. The analytes were extracted from the dust by sonication in acetone.
The extracts were solvent-exchanged to cyclohexane, frozen, thawed, and centrifuged prior to SEC injection. Following SEC, the eluent was split into 2 fractions, concentrated, and injected on-column into the gas chromatograph. This method represents the first complete publication describing
the SEC cleanup of organophosphorus pesticides in dusts. Recoveries of pesticides in dusts ranged from 63.5–110.8 ± 4.9–19.6% over a fortification range of 0.20–10.00 μg/g. This optimized, automated, and reproducible SEC method does not require further treatment
or cleanup for trace determination of these organophosphorus pesticides.
Document Type: Research Article
University of Washington, Environmental Health Laboratory, Box 357234, Seattle, WA 98195-7234; SNBL USA, Merrill Cr. Pkwy, Everett, WA 98203.
University of Washington, Environmental Health Laboratory, Box 357234, Seattle, WA 98195-7234.
University of Washington, Environmental Health Laboratory, Box 357234, Seattle, WA 98195-7234; Oregon Health Sciences University, Center for Research on Occupational and Environmental Toxicology, Portland, OR.
Publication date: January 1, 2002
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