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Residues of oxytetracycline (OTC) in edible tissues (muscle, liver, and kidney) of 18 turkeys were determined after continuous administration of the drug for 3 days in drinking water at the maximum recommended concentration of 400 mg/L. The European Union (EU) maximum residue limits
(MRLs) set for OTC are 100 μg/kg in muscle tissues, 300 μg/kg in liver, and 600 μg/kg in kidney, as the sum of the parent compound and its derivative 4′-epi-oxytetracycline (4-epi-OTC). Cleanup of tissue samples was performed by metal chelate affinity chromatography (MCAC),
but the original technique was miniaturized by the adoption of a mini solid-phase extraction column, allowing reduction of solvents, time, and hazardous waste. OTC and its 4′-epimer were quantitated by an isocratic liquid chromatography elution with UV detection. After 1 day of withdrawal,
OTC plus 4-epi-OTC residues were greater than MRL values in muscle and liver; 3 days after the end of treatment, all tissue residues were far lower than the MRL values. At the first day after the end of treatment, 4-epi-OTC was detected at very low concentrations only in muscle, in liver after
1 and 3 days of withdrawal, and in kidney at all sampling times. The withdrawal time was calculated according to EU recommendations and was set at 5 days.
Document Type: Research Article
Università degli Studi di Padova, Istituto di Patologia e Igiene Veterinaria, Agripolis Legnaro Padova, Italy. 2:
Università degli Studi di Bologna, Dipartimento di Sanità Pubblica e Patologia Animale, Via Tolara di Sopra 50, Ozzano Emilia Bologna, Italy. 3:
Servizi Veterinari A.S.L.10, S.Donà di Piave, Venezia, Italy.
Publication date: January 1, 2002
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The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.