Residues of Oxytetracycline and Its 4′-Epimer in Edible Tissues from Turkeys
Abstract:Residues of oxytetracycline (OTC) in edible tissues (muscle, liver, and kidney) of 18 turkeys were determined after continuous administration of the drug for 3 days in drinking water at the maximum recommended concentration of 400 mg/L. The European Union (EU) maximum residue limits (MRLs) set for OTC are 100 μg/kg in muscle tissues, 300 μg/kg in liver, and 600 μg/kg in kidney, as the sum of the parent compound and its derivative 4′-epi-oxytetracycline (4-epi-OTC). Cleanup of tissue samples was performed by metal chelate affinity chromatography (MCAC), but the original technique was miniaturized by the adoption of a mini solid-phase extraction column, allowing reduction of solvents, time, and hazardous waste. OTC and its 4′-epimer were quantitated by an isocratic liquid chromatography elution with UV detection. After 1 day of withdrawal, OTC plus 4-epi-OTC residues were greater than MRL values in muscle and liver; 3 days after the end of treatment, all tissue residues were far lower than the MRL values. At the first day after the end of treatment, 4-epi-OTC was detected at very low concentrations only in muscle, in liver after 1 and 3 days of withdrawal, and in kidney at all sampling times. The withdrawal time was calculated according to EU recommendations and was set at 5 days.
Document Type: Research Article
Affiliations: 1: Università degli Studi di Padova, Istituto di Patologia e Igiene Veterinaria, Agripolis Legnaro Padova, Italy. 2: Università degli Studi di Bologna, Dipartimento di Sanità Pubblica e Patologia Animale, Via Tolara di Sopra 50, Ozzano Emilia Bologna, Italy. 3: Servizi Veterinari A.S.L.10, S.Donà di Piave, Venezia, Italy.
Publication date: January 1, 2002
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