Determination of Ephedrine-Type Alkaloids in Dietary Supplements by LC/MS Using a Stable-Isotope Labeled Internal Standard

Authors: Gay, Martha L.1; White, Kevin D.1; Obermeyer, William R.2; Betz, Joseph M.3; Musser, Steven M.1

Source: Journal of AOAC International, Volume 84, Number 3, May 2001 , pp. 761-769(9)

Publisher: AOAC International

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Abstract:

Arapid, isocratic method for the separation of 6 ephedrine-type alkaloids which may occur in dietary supplements was modified to permit quantitation and confirmation by liquid chromatography/mass spectrometry using atmospheric pressure chemical ionization. Several columns and sets of conditions were examined for suitability and the results compared. The use of ion-pairing reagents to enhance resolution is also reported. Mass spectrometric conditions were adjusted to provide sufficient fragmentation for qualitative identification of the analytes. Stable-isotope labeled ephedrine was used as an internal standard for quantitation.

Document Type: Research Article

Affiliations: 1: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Washington, DC 20204. 2: ConsumerLab.com, 1234 Rock Hill Rd, Pasadena, MD 21122. 3: American Herbal Products Association, 8484 Georgia Ave, Suite 370, Silver Spring, MD.

Publication date: May 1, 2001

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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