Voltammetric Behavior and Analytical Applications of Lomefloxacin, an Antibacterial Fluorquinolone
Abstract:Lomefloxacin was reduced on a dropping mercury electrode, producing one or more peaks, depending on the pH of the aqueous medium. Coulometric measurements gave an experimental value of 1 electron for the main peak. Electrolysis was followed by UV spectrophotometry and liquid chromatography (LC), showing that a new band at 413 nm appeared for the electrolysis product in an acidic medium. Furthermore, by using UV spectrophotometry, an apparent pKa value of 6.75 ± 0.05 was obtained for lomefloxacin corresponding to the carboxyl moiety in the 3-position. For analytical studies, the differential pulse polarographic mode in 0.1N HCl was selected. The repeatability and reproducibility of the method were adequate (coefficient of variation [CV], 0.51%). The calibration curve method was used for the lomefloxacin concentration range of 7.0 × 10−6 to 7.0 × 10−5M. The detection and quantitation limits were 1.0 × 10−6 and 6.9 × 10−6M, respectively. For purposes of comparison, both UV spectrophotometric and LC (with UV and fluorimetric detection) methods were developed. The polarographic method showed good selectivity with respect to both excipients and degradation products. The recovery study showed a CV of <2% and an average recovery of 99.5% and it was not necessary to treat the sample before analysis. The method was applied to the determination of the uniformity content of lomefloxacin commercial tablets. The polarographic method was also successfully applied to the quantitation of lomefloxacin in urine, and the renal excretion profile was also determined.
Document Type: Research Article
Affiliations: University of Chile, Chemical and Pharmaceutical Sciences Faculty, Bioelectrochemistry Laboratory, PO Box 233, Santiago 1, Chile.
Publication date: May 1, 2001
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- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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