Authors: Rastogi, Vipin K.; Wallace, Lalena; Smith, Lisa S.

Source: Military Medicine, Volume 172, Number 11, November 2007 , pp. 1166-1169(4)

Publisher: AMSUS - Association of Military Surgeons of the U.S.

Abstract:

The efficacy of ultraviolet C (UVC) light (100-280 nm) in the decontamination of three hospital-related surfaces, namely, unpainted/painted aluminum (bed railings), stainless steel (operating tables), and scrubs (laboratory coats), was investigated. Acinetobacter baumannii cells were inoculated (10⁵ or 10³ cells) on small coupons and dried overnight in a class II biosafety cabinet. Drying resulted in ≤50% loss of viability. The UVC fluence of 90 J/m² was observed to be very effective in the decontamination of cells from all metal coupon surfaces (complete killing). However, the same fluence was ineffective in the decontamination of scrubs. The effectiveness of two other common disinfection practices, that is, 15 minutes of boiling or spraying with 70% ethanol, was investigated for the scrubs. Although ethanol treatment was ineffective, the boiling treatment was very effective (complete killing). These results establish that metal surfaces can be decontaminated with UVC irradiation and boiling treatment is effective for scrub decontamination.

Document Type: Research article

Publication date: 2007-11-01

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• Military Medicine is the Association's official monthly journal. The objective of the Journal is to promote awareness of Federal medicine by providing a forum for responsible discussion of common ideas and problems relevant to Federal healthcare. Its mission is: To increase healthcare education by providing scientific and other information to its readers; to facilitate communication; and to offer a prestige publication for members' writings.
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