Forty-five broiler arcasses from 6 different flocks were condemned due to liver lesions at processing meat inspection, and collected for pathological and bacterial examination. All affected chickens showed liver enlargement with distrahepatic bile ducts contained yellow inspissated
cream-coloured material. Histopathologically, extensive proliferation of bile ductules with fibrosis was observed in interlobular connective tissue, and it spread to form bridges with adjoining triads. Destruction and obstruction of portal bile ducts with multiple granulomas due to bacterial
infection and outflow of the bile were frequently observed.Many-Gram-positive bacilli were seen in the lesions, and they were identified asClostridium perfringensby indirect immunofluores en ecstaining technique.Clostridium perfringenswas isolated from affected livers. These findings are consistent
with cholangiohepatitis. Therefore, it is suggested thatC. perfringensmight be important in the pathogenesis of cholangiohepatitis in broiler chickens.