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Seed health testing of corn for Cephalosporium maydis

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In seed health testing of 13 corn cultivars,potato dextrose yeast extract agar medium showed to be more efficient than blotters in detecting C.maydis .Detection of the fungus started after two days of incubation at 20 şC under 12 hours alternating cycles of near ultraviolet light (NUV)and darkness.The fungus was distinguished in 12 seed samples on agar medium,however only nine samples were detected on blotters.Maximum infection rate in seed sample of Giza 2 cultivar reached up to 9%on agar medium compared with 5%on blotters.C.maydis was detected in a relatively higher percentage in white corn seeds (1 –9%)than in yellow cultivars (1 –3%).C.maydis was detected in different ear parts,i.e.,ear branch,cob, seeds,ear husks and silk of naturally infected corn cultivars.It was mostly manifested in the branch followed by cob,seeds,husks and silk.C.maydis is internally and externally seed-borne in corn as it was detected in relatively high percentage in the embryo and in both endosperm and coat of the 13 tested seed samples,with the exception of seeds of Amon cultivar in which the fungus was confined only to the seed coat.
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Document Type: Research Article

Publication date: 1999-12-10

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