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High incidence of hop stunt viroid in Hungarian grapevines

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The 297 nucleotide sequence of HSVd gvkr,a hop stunt viroid (HSVd gvkr)variant present in the Hungarian grapevines was determined from cDNAs amplified by the polymerase chain reaction.HSVd gvkr is very similar in its nucleotide sequences to several HSVd isolates previously detected in cucumber Citrus and grapevine.It exhibits 97.6%sequence homology with a HSVd grapevine isolate from Germany. HSVd gvkr isolate sequence was included in the phylogenic tree of different hop stunt viroid sequences. Using dot blot detection technique an intensive survey was performed in different Hungarian vineyards and a high incidence of this viroid was observed.More than 90%of the samples taken from three independent vineyards were positive for the HSVd gvkr probe.The wide distribution of this viroid could be attributed to the fact that grapevine nurseries were not screened before for HSVd.

Document Type: Research Article

Publication date: 1999-12-10

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