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Cabbage infected with phytoplasma from the aster yellows group in Hungary

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In 1997 and 1998 diseased cabbages (Brassica oleracea var .capitata)showing symptoms of possible phytoplasma infection were collected from several regions in Hungary.The diseased cabbages, showed shorter,thicker leaves and a long thick shoot.The leaves grew outward from the apex,not forming a cabbage head.Infection occurring after the formation of the cabbage head resulted in early flowering.The plants were studied to see whether they were infected by phytoplasmas.Polymerase chain reaction (PCR) using the P1/P7 universal phytoplasma primer pair showed the cabbage to be infected with phytoplasmas. The PCR amplified product of about 1.8 kbp,a segment of the phytoplasma 16S rDNA,was digested with Alu I and Rsa I to determine which strain cluster the infecting agent belonged to.The restriction fragment length polymorphism (RFLP)profiles obtained from the digestion suggested that the phytoplasma belonged to the American aster yellow (AAY)strain cluster.
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Document Type: Research Article

Publication date: 1999-12-10

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