IngentaConnect Adhesion molecules and their ligands in nasal polyps of aspirin-h...

Authors: Kupczyk, Maciej¹; Kupryś, Izabela¹; Danilewicz, Marian²; Bocheńska-Marciniak, Małgorzata¹; Murlewska, Anna³; Górski, Paweł¹; Kuna, Piotr¹

Source: Annals of Allergy, Asthma and Immunology, Volume 96, Number 1, January 2006 , pp. 105-111(7)

Publisher: American College of Allergy, Asthma, & Immunology

Abstract:

Background: Chronic inflammation with tissue eosinophilia plays a key role in the pathogenesis of asthma and nasal polyps in patients with aspirin hypersensitivity.

Objective: To evaluate the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) and their ligands (the integrins lymphocyte function-associated antigen 1 and very late-activation antigen 4 [VLA-4]) in nasal polyps of patients with aspirin hypersensitivity compared with aspirin-tolerant individuals.

Methods: Immunohistochemical studies were performed using a peroxidase method and monoclonal antibodies on 6-μm-thick cryostat sections cut from frozen polyps collected during elective surgery from 21 aspirin-sensitive and 23 aspirin-tolerant patients.

Results: The mean ± SD values of the semiquantitatively evaluated immunoexpression of ICAM-1, VCAM-1, and VLA-4 were significantly increased in patients with aspirin hypersensitivity compared with aspirin-tolerant patients (1.7 ± 0.8 vs 0.9 ± 0.8, P < .003; 1.8 ± 0.8 vs 0.8 ± 0.8, P < .001; and 2.2 ± 0.7 vs 1.3 ± 0.7, P < .001, respectively), whereas the mean ± SD values of the expression of lymphocyte function-associated antigen 1 did not differ significantly (2.4 ± 0.5 vs 2.2 ± 0.9; P = .57). We found a correlation between the immunoexpression of VCAM-1 and its ligand VLA-4 in all studied tissue samples (r = 0.4; P < .02).

Conclusions: In nasal polyps of aspirin-hypersensitive patients, up-regulation of the adhesion molecules ICAM-1 and VCAM-1 and the integrin VLA-4 may play an important role in the development of chronic eosinophilic inflammation.

Document Type: Original article

Affiliations: 1: Division of Pneumonology and Allergy, Department of Internal Medicine, Medical University of Łódź, Łódź, Poland. 2: Division of Morphometry, Department of Nephropathology, Medical University of Łódź, Łódź, Poland. 3: Department of Otorhinolaryngology, Medical University of Łódź, Łódź, Poland.

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Adhesion molecules and their ligands in nasal polyps of aspirin-hypersensitive patients