Children With Severe Early Childhood Caries: Pilot Study Examining Mutans Streptococci Genotypic Strains After Full-mouth Caries Restorative Therapy

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Abstract:

Purpose: Genotypic strains of mutans streptococci (MS) may vary in important virulence properties and be differentially affected by specific components of full-mouth caries restorative therapy. The purpose of this pilot study was to identify mutans streptococci strains that predominate following caries restorative therapy. Methods: Plaque from 7 children with severe early childhood caries was collected before and following therapy. MS isolates (N=828) were subjected to polymerase chain reaction (PCR) and arbitrarily primed-PCR (AP-PCR) for assignment within MS strains. Determining the longitudinal changes in MS strain distribution over time within each patient required the isolation of larger numbers of isolates per patient, but from fewer patients. Results: Up to 39 genotypic strains of Streptococcus mutans and Streptococcus sobrinus, and 7 genotypic strains of non-MS streptococci were identified by AP-PCR and 16S ribosomal rRNA gene sequencing. The number of MS strains isolated from each patient were 3 to 7 prior to treatment, diminishing to 1 to 2 dominant MS strains in most patients 6 months following therapy. Conclusions: Caries restorative therapy resulted in shifts of specific mutans streptococcus and non-mutans streptococcus strains. The implications are that caries restorative therapy affects the distribution of MS strains, and that well-accepted practices for caries prevention should be more closely examined for efficacy.

Keywords: CARIOLOGY; GENETICS; MICROBIOLOGY

Document Type: Research Article

Affiliations: 1: Pediatric Dentistry, Department of Pediatric Dentistry, Oregon Health & Science University (OHSU), Portland, Ore, USA 2: Integrative Bio-sciences, OHSU, Portland, Ore, USA 3: Department of Pediatric Dentistry, Oregon Health & Science University (OHSU), Portland, Ore, USA 4: OHSU, Portland, Ore, USA 5: private practice, Southern California, USA 6: Private practitioner in dentistry, Portland, Ore, USA 7: Oregon Clinical and Translational Research Institute and Division of Bioinformatics and Computational Biology, Department of Medical Informatics and Clinical Epidemiology, OHSU, USA 8: Integrative Biosciences and Oral Pathology and Radiology, OHSU, Portland, Ore, USA 9: Integrative Biosciences and Pediatric Dentistry, OHSU, Portland, Ore, USA. machidac@ohsu.edu

Publication date: March 1, 2012

More about this publication?
  • Pediatric Dentistry is the official publication of the American Academy of Pediatric Dentistry, the American Board of Pediatric Dentistry and the College of Diplomates of the American Board of Pediatric Dentistry. It is published bi-monthly and is internationally recognized as the leading journal in the area of pediatric dentistry. The journal promotes the practice, education and research specifically related to the specialty of pediatric dentistry. This peer-reviewed journal features scientific articles, case reports and abstracts of current pediatric dental research.
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