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Application of Adenosine Triphosphate-Driven Bioluminescence for Quantification of Plaque Bacteria and Assessment of Oral Hygiene in Children

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Abstract:

Purpose: Dentistry has undergone a shift in caries management toward prevention and improved oral hygiene and diagnosis. Caries prevention now represents one of the most important aspects of modern dental practice. The purpose of this cross-sectional study was to demonstrate the use of adenosine triphosphate- (ATP-) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plague streptococci) and assessment of oral hygiene and caries risk. Methods: Thirty-three pediatric patients (7- to 12-year-old males and females) were examined, and plague specimens, in addition to stimulated saliva, were collected from representative teeth within each quadrant. Oral specimens (n=150 specimens) were assessed by plating on enriched and selective agars, to enumerate total bacteria and streptococci, and subjected to adenosine triphosphate- (ATP-) driven bioluminescence determinations using a luciferase-based assay system. Results: Statistical correlations, linking ATP values to numbers of total bacteria, oral streptococci and mutans streptococci, yielded highly significant r values of 0.854, 0.840, and 0.796, respectively Conclusions: Our clinical data is consistent with the hypothesis that ATP measurements have a strong statistical association with bacterial number in plague and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene and caries risk in children.

Keywords: ATP-DRIVEN BIOLUMINESCENCE; CARIES RISK ASSESSMENT; ORAL HYGIENE; PEDIATRIC PATIENTS; PLAQUE BACTERIA

Document Type: Research Article

Affiliations: 1: Resident in Pediatric Dentistry, Oregon Health & Science University, Portland, Ore; Private practice in Los Altos, Calif 2: Research assistant in Integrative Biosciences; School of Medicine, Oregon Health & Science University, Portland, Ore 3: School of Dentistry, Oregon Health & Science University, Portland, Ore 4: Resident in Pediatric Dentistry, School of Dentistry, Oregon Health & Science University, Portland, Ore 5: Pediatric Dentistry, School of Dentistry, Oregon Health & Science University, Portland, Ore 6: Integrative Biosciences and Oral Pathology and Radiology, School of Dentistry, Oregon Health & Science University, Portland, Ore 7: Professor of Integrative Biosciences and Pediatric Dentistry, School of Dentistry, Oregon Health & Science University, Portland, Ore

Publication date: May 1, 2010

More about this publication?
  • Pediatric Dentistry is the official publication of the American Academy of Pediatric Dentistry, the American Board of Pediatric Dentistry and the College of Diplomates of the American Board of Pediatric Dentistry. It is published bi-monthly and is internationally recognized as the leading journal in the area of pediatric dentistry. The journal promotes the practice, education and research specifically related to the specialty of pediatric dentistry. This peer-reviewed journal features scientific articles, case reports and abstracts of current pediatric dental research.
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