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Open Access Repeated Administration of Tribromoethanol in C57BL/6NHsd Mice

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Abstract:

We evaluated the effect of repeated intraperitoneal administration of tribromoethanol on various parameters in C57BL/6NHsd mice. Mice (n = 68) were randomly assigned to 1 of 7 groups to receive tribromoethanol (500 mg/kg IP) on day 0 or days 0 and 8; vehicle (tert-amyl alcohol in sterile water) only on day 0 or days 0 and 8; sterile water injection on day 0 or days 0 and 8; or no treatment. A single dose of tribromoethanol failed to produce loss of pedal reflex and had no effect on median food and water consumption but altered median body weight on days 1 through 4 when compared with that in mice that received vehicle only or no treatment. Median body weight did not differ between mice that received a single dose of tribromoethanol and those that received an injection of water. Among mice given 2 doses of tribromoethanol, induction time, anesthetic duration, and recovery time varied widely. Repeated administration of tribromoethanol had no effect on median food and water consumption or body weight compared with those in controls. Median liver weight was significantly greater in mice that received 2 doses compared with a single dose of tribromoethanol. Median liver weight did not differ between untreated mice and those that received tribromoethanol. No significant organ or tissue pathology was observed in any study animal. Although tribromoethanol did not produce morbidity, mortality, or pathologic changes in treated animals, we urge caution in use of tribromoethanol in C57BL/6NHsd mice due to its variable anesthetic effectiveness.

Document Type: Research Article

Affiliations: 1: Departments of Biomedical and Diagnostic Sciences, Office of Laboratory Animal Care, University of Tennessee, Knoxville, Tennessee, USA. wahill@utk.edu 2: Department of Laboratory Animal Resources, Duke University, Durham, North Carolina, USA 3: Department of Office of Laboratory Animal Care, University of Tennessee, Knoxville, Tennessee, USA 4: Department of Biomedical and Diagnostic Sciences, University of Tennessee, Knoxville, Tennessee, USA 5: Department of Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA 6: Department of Small Animal Clinical Sciences, Institute of Agriculture, Knoxville, University of Tennessee, Knoxville, Tennessee, USA

Publication date: 2013-03-01

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  • The Journal of the American Association for Laboratory Animal Science (JAALAS) serves as an official communication vehicle for the American Association for Laboratory Animal Science (AALAS). The journal includes a section of refereed articles and a section of AALAS association news. The mission of the refereed section of the journal is to disseminate high-quality, peer-reviewed information on animal biology, technology, facility operations, management, and compliance as relevant to the AALAS membership. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles, including solicited manuscripts, must include appropriate references and must undergo peer review.

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