A New Method for Respiratory Gating During Microcomputed Tomography of Lung in Mice
Authors: Johnson, Evan M.; Price, Roger E.; Kurie, Jonathan M.; Rivera, Belinda S.; Cody, Dianna D.
Source: Journal of the American Association for Laboratory Animal Science, Volume 47, Number 4, July 2008 , pp. 46-56(11)
Publisher: American Association for Laboratory Animal Science
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Abstract:
This study investigated the use of regulated cyclic breath-holds to improve microcomputed tomography (μCT) imaging of small (diameter, less than 1 mm) mouse lung tumors in vivo. Two novel techniques that use a modified small-animal ventilator were examined and compared with a previously used respiratory gating μCT technique and a free-breathing μCT technique. Two mice were scanned with each of these 4 μCT techniques (voxel size, 92 μm). The appearance of small lung tumors (maximal diameter, 0.5 to 1.0 mm) and the characteristics of line profiles of the lung-diaphragm boundary were used to compare the images obtained from the 4 acquisition techniques. The use of cyclic breath-holds, synchronized with the CT exposures, led to marked improvement in the visualization of the mouse lung structure and lesion conspicuity. A secondary experiment was performed to assess the stress placed on mice by the acquisition techniques.Document Type: Research article
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