Comparison of Technicians' Ability to Detect Clinical Signs in Rats Housed in Wire-bottom versus Solid-bottom Cages with Bedding
Abstract:Rodent toxicology studies have historically been performed in wire-bottom cages, but the 1996 Guide for the Care and Use of Laboratory Animals recommends solid-bottom caging with bedding. Some investigators have expressed concern that changing to solid-bottom cages would interfere with technicians' ability to detect clinical signs. To test this hypothesis, rats were housed in both types of caging and given compounds to induce a variety of subtle clinical signs common to toxicology studies including chromodacryorrhea, soft stool, stereotypic behaviors, mild hypoactivity, abnormal postures, and discolored urine. For one comparison, fecal pellets were removed to simulate decreased production of feces. Technicians, blinded from knowing which animals had been treated, observed the rats and recorded the clinical signs they detected. The technicians who administered the treatments verified that clinical signs were present before and after the blinded technicians made their observations. The number of animals observed with clinical signs divided by the number of animals verified with signs was calculated for each compound and compared between the cage types by using the Fisher Exact Test. The only statistically significant difference observed was a diminished ability to detect discolored, dark urine from rats in wire-bottom cages. These results suggest that concerns about technical staff's inability to detect clinical signs in toxicity tests should not prevent investigators from using solid-bottom cages with bedding.
Document Type: Research Article
Publication date: March 1, 2008
The Journal of the American Association for Laboratory Animal Science (JAALAS) serves as an official communication vehicle for the American Association for Laboratory Animal Science (AALAS). The journal includes a section of refereed articles and a section of AALAS association news. The mission of the refereed section of the journal is to disseminate high-quality, peer-reviewed information on animal biology, technology, facility operations, management, and compliance as relevant to the AALAS membership. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles, including solicited manuscripts, must include appropriate references and must undergo peer review.
Attention Members: To access the full text of the articles, be sure you are logged in to the AALAS website.
Attention: please note, due to a temporary technical problem, reference linking within the content is not available at this time
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- For issues prior to 1997
- Institutional Subscription Activation
- ingentaconnect is not responsible for the content or availability of external websites