Minipigs as an Animal Model for Dermal Vaccine Delivery
Abstract:Appropriate animal models for intradermal vaccine delivery are scarce. Given the high similarity of their skin anatomy to that of humans, minipigs may be a suitable model for dermal vaccine delivery. Here we describe the immunization of Göttingen minipigs by using intradermal and intramuscular delivery of hepatitis B surface antigen (HBsAg). Intradermal vaccine delivery by needle and syringe and by needle-free jet injection induced humoral antiHBsAg responses. Priming immunization by using the disposable syringe jet injector (DSJI) resulted in a higher antibody titer than did conventional intradermal immunization and a titer comparable to that after intramuscular vaccination with HBsAg and Al(OH)3 adjuvant. This study highlights the utility of the minipig model in vaccine studies assessing the efficacy of conventional and novel methods of dermal delivery. Moreover, we include suggestions regarding working with minipigs during dermal vaccine delivery studies, thereby fostering future work in this area of vaccinology.
Document Type: Research Article
Affiliations: 1: Institute for Translational Vaccinology, Bilthoven, The Netherlands. firstname.lastname@example.org 2: National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands 3: Institute for Translational Vaccinology, Bilthoven, The Netherlands 4: Ellegaard Göttingen Minipigs, Dalmose, Denmark 5: PharmaJet, Golden, Colorado, Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands 6: Institute for Translational Vaccinology, Bilthoven, The Netherlands; Division of Drug Technology, Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands
Publication date: February 1, 2014
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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