Assessing the Pulsatility of Luteinizing Hormone in Female Vervet Monkeys (Chlorocebus aethiops sabaeus)

Authors: Stephens, Sahar M1; Pau, Francis KY2; Yalcinkaya, Tamer M3; May, Margaret C4; Berga, Sarah L5; Post, Miriam D6; Appt, Susan E7; Polotsky, Alex J8

Source: Comparative Medicine, Volume 63, Number 5, October 2013 , pp. 432-438(7)

Publisher: American Association for Laboratory Animal Science

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Abstract:

Specific alterations in the pulsatility of luteinizing hormone (LH) are linked to obesity-related subfertility in ovulatory women. Vervet monkeys (Chlorocebus aethiops sabaeus) are an Old World nonhuman primate that develops obesity and has a menstrual cycle similar to humans. We evaluated follicular-phase LH pulses in 12 adult normal-weight female vervets. Serum was collected every 10 min for 4 h by using a tether device in conscious, freely moving monkeys on menstrual cycle days 2 through 5. Serum estradiol was collected daily during the follicular phase to identify the luteal–follicular transition. For comparison, we used data from 12 ovulatory normal-weight women who had undergone frequent blood sampling of early-follicular LH. LH pulse frequency was similar, with 2.8 ± 0.7 LH pulses during 4 h in vervets compared with 2.3 ± 0.7 LH pulses during 4 h in women. The LH pulse mass (percentage change in the pulse peak over the preceding nadir) was 123.2% ± 27.4% in vervets and 60.9% ± 14.9% in humans. The first day of low serum estradiol after the follicular-phase peak was denoted as the day of the luteal–follicular transition. Luteectomy was performed on luteal days 7 through 9, and corpora lutea were confirmed by histology. We demonstrate that follicular LH patterns in vervets are similar to those in humans and that the luteal phase is easily identified by monitoring daily serum estradiol. These findings demonstrate that vervet monkeys are a suitable animal model for evaluating LH pulse dynamics longitudinally in studies of diet-induced obesity.

Keywords: CL; CORPUS LUTEUM; LH; LUTEINIZING HORMONE

Document Type: Research Article

Affiliations: 1: Reproductive Endocrinology, University of Colorado Denver, Denver, Colorado, USA 2: Oregon National Primate Research Center, Beaverton, Oregon, USA 3: department of Obstetrics and Gynecology, Wake Forest School of Medicine and Primate Center, Winston-Salem, North Carolina, USA 4: Department of Pathology/Comparative Medicine, Wake Forest School of Medicine and Primate Center, Winston-Salem, North Carolina 5: Department of Obstetrics and Gynecology, Wake Forest School of Medicine and Primate Center, Winston-Salem, North Carolina, USA 6: Department of Pathology, University of Colorado Denver, Denver, Colorado, USA 7: Department of Pathology/Comparative Medicine, Wake Forest School of Medicine and Primate Center, Winston-Salem, North Carolina, USA 8: Department of Reproductive Endocrinology, University of Colorado Denver, Denver, Colorado, USA. alexpolotsky@ucdenveredu

Publication date: October 1, 2013

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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