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Open Access Predictive Observation-Based Endpoint Criteria for Mice Receiving Total Body Irradiation

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Abstract:

Total body irradiation of mice is a commonly used research technique; however, humane endpoints have not been clearly identified. This situation has led to the inconsistent use of various endpoints, including death. To address this issue, we refined a cageside observation-based scoring system specifically for mice receiving total body irradiated. Male and female C57BL/6 mice (age, 8 wk) received 1 of 3 doses of radiation from 1 of 2 different radiation sources and were observed for progression of clinical signs. All mice were scored individually by using cageside observations of their body posture (score, 0 to 3), eye appearance (0 to 3), and activity level (0 to 3). Retrospective analysis of the observation score data indicated that death could be predicted accurately with total scores of 7 or greater, and observation scores were consistent between observers. This scoring system can be used to increase the consistent use of endpoint criteria in total body murine irradiation studies and ultimately to improve animal welfare.

Document Type: Research Article

Affiliations: 1: Biologic Resources Laboratory, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA; MED Institute, West Lafayette, Indiana, USA. lizzynun@gmail.com 2: School of Public Health, Division of Epidemiology and Biostatistics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA 3: Biologic Resources Laboratory, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA 4: Toxicology Research Laboratory, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA. Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA

Publication date: August 1, 2013

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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