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Open Access tmie Is Required for Gentamicin Uptake by the Hair Cells of Mice

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The circling (cir/cir) mouse is a spontaneous model of deafness due to deletion of a 40-kb genomic region that includes the transmembrane inner ear (tmie) gene. In addition to being deaf, cir/cir mice exhibit abnormal behaviors including circling and hyperactivity. Here we investigated differences between 3-d-old (that is, before hair-cell degeneration) cir/cir and phenotypically normal (+/cir) mice and the reason underlying the degeneration of the inner ear structure of cir/cir mice. To this end, we used gentamicin, gentamicin–Texas red conjugate, and FM1-43 to investigate mechanotransducer channel activity in the hair cells of cir/cir mice; these compounds are presumed to enter hair cells through the mechanotransducer channel. Although the structure of the inner ear of +/cir mice was equivalent to that of cir/cir mice, the hair cells of cir/cir mice (unlike +/cir) did not take up gentamicin, gentamicin–Texas red conjugate, or FM1-43. These findings suggest that hair cells in cir/cir mice demonstrate abnormal maturation and mechanotransduction. In addition, our current results indicate that tmie is required for maturation and maintenance of hair cells.
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Document Type: Case Report

Affiliations: 1: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, Korea 2: Center for Neuroscience, Department of Anesthesiology and Pain Medicine, Program in Communication Science, School of Medicine, University of California, Davis, California, USA 3: Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea 4: Department of Otolaryngology, College of Medicine, Kyungpook National University, Daegu, Korea 5: College of Ecology and Environmental Science, Animal BT Science Laboratory of Developmental and Differenciational Regulation, Kyungpook National University, Daegu, Korea 6: Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea 7: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, Korea. [email protected]

Publication date: 2013-04-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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