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Open Access Cloning of Porcine Platelet Glycoprotein Ibα and Comparison with the Human Homolog

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Abstract:

Glycoprotein Ib–IX–V (GPIb–IX–V) is a platelet adhesion receptor complex that initiates platelet aggregation. Glycoprotein Ibα (GPIbα) is the central component of the GPIb–IX–V complex, anchoring the complex to the cytoskeleton and harboring the binding site for von Willebrand factor (vWF). Previous studies suggest that the coagulation function in pigs differs from that in humans, especially with respect to the interaction between vWF and platelets. However, we have little knowledge about the function of porcine platelets, which is important with regard to studies of cardiovascular disease, clotting, and surgery that use pigs as animal models. To extend this information, we cloned and analyzed the porcine GPIbα sequence. Porcine GPIbα contains 1891 nucleotides and includes an open reading frame that encodes 627 amino acids. The nucleotide sequence showed 67% identity with human GPIbα, whereas the deduced amino acid sequences were 59% identical. The vWF binding domain shares the highest identity among different species, whereas the PEST domain shows variations. Evaluation of platelet function by using ristocetin-induced platelet aggregation revealed remarkably lower levels of aggregation in porcine than human platelets. According to the sequence analysis and platelet aggregation tests, we propose that the function of GPIbα, especially regarding the ristocetin–vWF–GPIbα interaction, differs between pigs and humans. This characterization of porcine GPIbα will enhance our knowledge of the porcine coagulation system.

Document Type: Research Article

Affiliations: 1: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China 2: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China; Australian Centre for Blood Diseases, Monash University, Melbourne, Australia 3: Australian Centre for Blood Diseases, Monash University, Melbourne, Australia 4: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China. younanchen@126.com 5: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China. jqcheng@scu.edu.cn

Publication date: 2012-08-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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