Effect of Prophylactic Supplementation with Grape Polyphenolics on Endotoxin-Induced Serum Secretory Phospholipase A2 Activity in Rats
Abstract:This study investigated whether dietary supplementation of polyphenolics-rich grape extract (GE) could attenuate endotoxin-induced serum secretory phospholipase A2 (sPLA2) activity, a modulator of inflammation. Male Sprague–Dawley rats were fed a control diet or the diet supplemented with polyphenolic-rich GE (100 or 300 mg/kg daily) for 3 wk prior to intraperitoneal injection of 3 or 15 mg/kg LPS. A fluorometric assay was used to measure serum sPLA2 activity during a 5-d period before and after LPS injection. Body weight, hematocrit, and serum C-reactive protein level were also measured. Administration of LPS induced a rapid increase in sPLA2 activity, which peaked 1 to 2 d after LPS injection and resolved to near-baseline values on days 4 to 5. Marked declines in body weight and hematocrit, increases in C-reactive protein levels, and effects on health status also occurred. GE supplementation significantly attenuated the LPS-induced increase in sPLA2 activity and decline in hematocrit, but its effects on the loss of body weight and C-reactive protein levels were not significant. Among the measurements, serum sPLA2 was the only marker that showed a dose-dependent response to both LPS and GE supplementation. The current findings show that oral consumption of polyphenolic-rich GE suppresses endotoxin-induced sPLA2 activity.
Document Type: Research Article
Affiliations: 1: Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Wisconsin - Madison, Madison, Wisconsin, USA. firstname.lastname@example.org 2: Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Wisconsin - Madison, Madison, Wisconsin, USA 3: Department of Surgery, Reed Research Group, Department of Animal Sciences, University of Wisconsin - Madison, Madison, Wisconsin, USA 4: Mucosal Immunology Research Core, Reed Research Group, Department of Animal Sciences, University of Wisconsin - Madison, Madison, Wisconsin, USA 5: Research Animal Resources Center, School of Medicine and Public Health, University of Wisconsin - Madison, Madison, Wisconsin, USA
Publication date: August 1, 2012
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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