Open Access Ulcerative Dermatitis in C57BL/6 Mice Lacking Stearoyl CoA Desaturase 1

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Abstract:

Ulcerative dermatitis (UD) is a common cause of morbidity and euthanasia in mice with a C57BL/6 (B6) background. The purposes of the current study were to determine whether UD lesions could be reliably produced in B6 mice lacking stearoyl-CoA desaturase 1 (SCD1–/– mice), to ascertain whether the UD lesions in SCD1–/– mice were similar to those found in other B6 mice, and to characterize the cell invasion phenotype of Staphlococcus xylosus cultured from the lesions. S. xylosus isolates from the environment and human skin were used as controls. SCD1–/– (n = 8 per group) and nontransgenic B6 control mice (n = 22 mice pooled from 3 groups that received different concentrations of conjugated linoleic acid) were fed standard rodent chow or a semipurified diet (NIH AIN76A) for 4 wk. Samples from other B6 mice with UD (field cases; n = 7) also were submitted for histology and culture. All of the SCD1–/– mice developed UD lesions by 4 wk on NIH AIN76A. None of SCD1–/– fed standard rodent chow and none of the wildtype B6 mice fed NIH AIN76A developed UD. Supplementation with conjugated linoleic acid did not affect ulcerogenesis. UD lesions in SCD1–/– mice and field cases were grossly and histologically similar. S. xylosus was isolated from SCD1–/– mice with UD (71%) and field cases of UD (43%). These isolates were the most cell-invasive, followed by the environmental isolate, and finally the human skin isolate. Our results provide a basis for further pathologic and clinical study of UD.

Document Type: Research Article

Affiliations: 1: Research Animal Resource Center, University of Wisconsin–Madison, Madison, Wisconsin, USA. lisakh@rarc.wisc.edu 2: Research Animal Resource Center, University of Wisconsin–Madison, Madison, Wisconsin, USA; Summit–Heritage Animal Clinics, Oconomowoc and Delafield, WI, USA. Globe University Veterinary Technologist Program, Madison, Wisconsin 3: Research Animal Resource Center, University of Wisconsin–Madison, Madison, Wisconsin, USA 4: Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin–Madison, Madison, Wisconsin, USA 5: Department of Animal Sciences, University of Wisconsin–Madison, Madison, Wisconsin, USA 6: School of Veterinary Medicine, University of Wisconsin–Madison, Madison, Wisconsin, USA 7: Department of Biochemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA 8: Department of Biochemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA; Department of Nutritional Sciences, University of Wisconsin–Madison, Madison, Wisconsin, USA

Publication date: August 1, 2012

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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