Genomic Analysis and Pathogenic Characteristics of Lymphocytic Choriomeningitis Virus Strains Isolated in Japan
Source: Comparative Medicine, Volume 62, Number 3, June 2012 , pp. 185-192(8)
Abstract:Lymphocytic choriomeningitis virus (LCMV) is a zoonotic pathogen of which mice are the natural reservoir. Different strains and clones of LCMV show different pathogenicity in mice. Here we determined the complete genomic sequences of 3 LCMV strains (OQ28 and BRC which were isolated from mice in Japan and WE(ngs) which was derived from strain WE). Strains OQ28 and BRC showed high sequence homology with other LCMV strains. Although phylogenetic analyses placed these 2 Japanese strains in different subclusters, they belonged to same cluster of LCMV isolates. WE(ngs) and WE had many sequence substitutions between them but fell into same subcluster. The pathogenicity of the 3 new LCMV isolates was examined by inoculating ICR mice with 102 and 104 TCID50 of virus. ICR mice infected with OQ28 or WE(ngs) exhibited severe clinical signs, and some of the infected mice died. In contrast, all ICR mice infected with BRC showed no clinical signs and survived infection. Virus was detected in the blood, organs, or both of most of the surviving ICR mice inoculated with either OQ28 or WE(ngs). However, virus was below the level of detection in all ICR mice surviving infection with strain BRC. Therefore, LCMV strains OQ28 and BRC were genetically classified in the same cluster of LCMV strains but exhibited very different pathogenicity.
Document Type: Research Article
Affiliations: 1: Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan; Quality Control Department, Bio Technical Center, Japan SLC, Hamamatsu, Japan 2: Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan 3: Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan 4: Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan; National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan
Publication date: 2012-06-01
- Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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