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Open Access Quantitation of Acute Phase Proteins and Protein Electrophoresis in Monitoring the Acute Inflammatory Process in Experimentally and Naturally Infected Mice

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Serologic screening for infectious disease in sentinel mice from rodent colonies is expensive and labor-intensive, often involving multiple assays for several different infectious agents. Previously, we established normal reference ranges for the protein fractions of several laboratory strains of mice by using a commercially available agarose system of protein electrophoresis. In the current study, we address protein fractionation and quantitation of acute phase proteins (APP) in mice experimentally infected with Sendai virus or mouse parvovirus. We further investigate this methodology by using samples from sentinel mice from colonies with endemic infection. All study groups showed significant increases in  globulins. Various other protein fractions showed mild variable changes; significant differences were not detected for individual APP. These results contrast the significant changes observed in APP and protein electrophoresis by using the standard methods of inducing inflammatory responses through injection of complete Freund adjuvant or LPS. These present data suggest that although quantitation of individual APP may not be helpful,  globulin levels may reflect infection in laboratory mice and provide a possible adjunct to traditional screening methods.

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Document Type: Research Article

Affiliations: 1: Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA. c.cray@miami.edu 2: University Animal Care, The University of Arizona, Tucson, Arizona 3: Trudeau Institute, Saranac Lake, New York 4: Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, Barry University, College of Health Sciences, Miami Shores, Florida 5: Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida 6: Division of Comparative Pathology, Department of Pathology, Division of Veterinary Resources, University of Miami Miller School of Medicine, Miami, Florida

Publication date: 2010-08-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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