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Open Access A Mouse Surgical Model for Metastatic Ovarian Granulosa Cell Tumor

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We recently described a genetically engineered mouse model that develops ovarian granulosa cell tumors (GCTs) that mimic many aspects of the advanced human disease, including distant dissemination. However, because the primary tumors killed their hosts before metastases were able to form, the use of these mice to study metastatic disease required the development of a simple, reliable, and humane surgical protocol for the excision of large GCTs from debilitated mice. Here we describe a protocol involving multimodal anesthesia, tumor removal through ventral midline celiotomy and perioperative fluid therapy, and analgesia that led to the postoperative survival of more than 90% of mice, despite the removal of tumors representing as much as 10% of the animal's body weight. Intraabdominal recurrence of the GCT did not occur in surviving animals, but most developed pulmonary or adrenal metastases (or both) by 12 wk after surgery. We propose that this mouse model of metastatic GCT will serve as a useful preclinical model for the development of novel treatment modalities and diagnostic techniques. Furthermore, our results delineate anesthetic and surgical principles for the removal of large abdominal tumors from mice that will be applicable to other models of human cancers.

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Document Type: Research Article

Affiliations: 1: Department of Clinical Sciences, College of Veterinary Medicine, University of Montréal, St Hyacinthe, Québec, Canada. 2: Department of Clinical Sciences, College of Veterinary Medicine, University of Montréal, St Hyacinthe, Québec, Canada 3: Department of Biomedicine Veterinary, College of Veterinary Medicine, University of Montréal, St Hyacinthe, Québec, Canada 4: Department of Comparative Medicine and Animal Resources Centre, McGill University, Montréal, Québec, Canada

Publication date: 2009-12-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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