Damage to Incisors after Nonmyeloablative Total Body Irradiation may Complicate NOD/SCID Models of Hemopoietic Stem Cell Transplantation
Abstract:Immunocompromised murine xenotransplantation models have become an important tool to study stem cell biology. One of the most common recipient strains used is the NOD/SCID mouse, which offers sufficient longevity to quantify moderate levels of engraftment. During pilot experiments, we noted incisor abnormalities 5 to 6 wk after nonmyeloablative doses of irradiation. Here we report a detailed examination of this phenomenon and propose possible explanations and management strategies. A total of 15 NOD/SCID mice received 3 Gy total body irradiation (TBI) and were monitored over 9 wk. A control group of 15 mice were treated in exactly the same way as the study mice except that they did not receive irradiation. A total of 9 TBI mice developed incisor abnormalities between days 40 and 50 after irradiation, resulting in rapid weight loss. No mice in the control group developed incisor abnormalities, however 3 were euthanized prematurely due to the development of thymic lymphoma. Upon development of incisor abnormalities and weight loss, 2 mice in the TBI group had their teeth trimmed and received soft food. Both mice made a rapid recovery and survived for the remainder of the study. The development of incisor abnormalities occurred in 2 substrains, and alterations in antibiotic use and supplementation of the vitamin content of feeds did not prevent the abnormalities. Investigators working with this model should be aware of this complication and modify protocols appropriately.
Document Type: Research Article
Publication date: June 1, 2006
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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