Evaluation of Two Combinations of Domitor, Zoletil 100, and Euthatal to Obtain Long-term Nonrecovery Anesthesia in Sprague-Dawley Rats
Abstract:We sought to evaluate a new protocol designed to maintain long-term, nonrecovery, surgical anesthesia in Sprague-Dawley rats. In the first phase, two groups of rats were anesthetized with two different dose combinations of Domitor (medetomidine) and Zoletil 100 (tiletamine–zolazepam) to investigate their efficacy in induction of anesthesia. One combination comprised Domitor at 35 g/kg and Zoletil 100 at 40 mg/kg, whereas the other comprised Domitor at 50 g/kg and Zoletil 100 at 20 mg/kg. Both combinations effectively induced deep anesthesia and caused no mortality, but the duration of anesthesia differed statistically. In the second phase, we induced anesthesia with both Domitor–Zoletil 100 dose combinations then investigated the possibility of maintaining anesthesia for 5 h by administering Euthatal (pentobarbitone) intra-arterially at 10 mg/kg hourly. Depth of anesthesia, mortality, physiological parameters, blood gas analysis, hematology, clinical chemistry, and postmortem histopathology were recorded. Euthatal provided stable long-term anesthesia with both dose combinations of Domitor–Zoletil 100. Seven of 8 (88%) animals anesthetized with Domitor at 50 g/kg and Zoletil 100 at 20 mg/kg successfully were maintained under deep anesthesia for 5 h. Higher mortality (36% versus 12%) occurred in group of animals treated with Domitor at 35 g/kg and Zoletil 100 at 40 mg/kg. This difference may be linked to dose-related respiratory depression, as alterations of arterial gas levels were noted. Our findings suggest that, when long-term nonrecovery anesthesia is required, doses of 50 g/kg Domitor and 20 mg/kg Zoletil 100 are preferable when given with Euthatal to maintain physiological conditions in animals.
Document Type: Research Article
Affiliations: 1: Laboratory Animal Science Department, GlaxoSmithKline Medicine, Research Centre, Via Fleming 4, 37135, Verona, Italy 2: Pathology Department, GlaxoSmithKline Medicine Research Centre, Via Fleming 4, 37135, Verona, Italy
Publication date: June 1, 2005
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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