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Open Access Survey of Captive Cynomolgus Macaque Colonies for SRV/D Infection Using Polymerase Chain Reaction Assays

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Abstract:

The exogenous simian type D retroviruses (SRV/Ds) are prevalent in macaque monkeys and sometimes cause immunodeficiency with anemia, weight loss, and persistent unresponsive diarrhea. SRV/D isolates are classified as subtypes 1 to 6, and the entire sequences of the gag region of SRV/D-1, -2, and -3 and SRV/D-Tsukuba (SRV/D-T) have been determined. We designed specific primers in the gag region of SRV/D-T that enabled us to directly detect by polymerase chain reaction (PCR) SRV/D-T proviral DNA sequences in DNA extracted from whole blood. Using this assay and another PCR assay that detects multiple SRV/D subtypes, we performed a survey for SRV/D infection in our specific pathogen-free (SPF) and conventional colonies at Tsukuba Primate Center (TPC). In the SPF colony, no SRV/D signal was detected in any animal. On the other hand, SRV/D-T was detected in 11 of 49 animals (22.5%) in the conventional colony. SRV/D-T was the only SRV/D subtype detected. Consequently, SRV/D-T is the major SRV/D subtype present in cynomolgus monkeys at TPC.

Document Type: Research Article

Affiliations: 1: Tsukuba Primate Center for Medical Science National Institute Infectious Diseases (NIID), 1 Hachimandai, Tsukuba, 305-0843 Japan 2: The Corporation for Production and Research of Laboratory Primates, 1 Hachimandai, Tsukuba, 305-0843 Japan 3: Graduate School of Life and Environmental Sciences, and Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Japan

Publication date: 2005-04-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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