Open Access Effect of Genetic Background on Cav2 Channel α1 and  Subunit Messenger RNA Expression in Cerebellum of N-Type Ca2+ Channel α1B Subunit-Deficient Mice

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Abstract:

Although the N-type Ca2+ channel plays a role in a variety of neuronal functions, N-type Ca2+ channel α1B-deficient mice exhibit normal life span without apparent behavioral or histologic abnormalities. To examine whether the reason for their normal behavior is compensation by other Cav2 channel α1 or  subunit genes and to analyze whether genetic background influences the subunit expression pattern, we studied the α1A, α1E, 1b, 2, 3 and 4 subunit mRNA levels in cerebellum of α1B-deficient mice with CBA × C57BL/6 or CBA/JN background. In cerebellum of the mice with a CBA × C57BL/6 background, α1A mRNA was expressed at a higher level than that in wild-type or heterozygous mice, but difference in the expression levels of α1E, 1b, 2, 3, and 4 subunits was not found among wild-type, heterozygous, and homozygous mice. In cerebellum of α1B-deficient mice with CBA/JN background, 4 mRNA was expressed at a higher level than that in wild-type or heterozygous mice, but α1A, α1E, 1b, α2, and 3 transcripts were expressed at similar levels in all genotypes. Therefore, a possible explanation of the normal behavior of α1B-deficient mice is that Cav2 channel family members compensate for the deficiency, and that the change of functional subunit expression pattern for compensation differs in animals with different genetic backgrounds.

Document Type: Research Article

Affiliations: 1: Tsukuba Research Laboratory, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan, Laboratory of Seeds Finding Technology, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan 2: Tsukuba Research Laboratory, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan

Publication date: December 1, 2004

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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