Immunocompetent Orthotopic Isograft Mouse Model of Ovarian Cancer for High-Intensity Focused Ultrasound (HIFU) Treatment
Abstract:Ovarian epithelial carcinoma is the most common form of ovarian cancer, causing more deaths than any other cancer in women with gynecologic malignancies in the United States. The poor outcome for women with late-stage ovarian cancer underscores the need for early detection strategies and for new treatment options to be developed. High-intensity focused ultrasound (HIFU) is a therapeutic modality that is safe and effective against various types of solid tumors, and has the potential to serve as a treatment for ovarian cancer. Use of an appropriate animal model is important in obtaining relevant data for translational research. The purpose of the study reported here was to modify and create an immunocompetent orthotopic tumor isograft model for evaluation of intra-operative HIFU application. This model would resemble the clinical presentation of human patients with late-stage ovarian cancer. We were able to consistently produce a surgical model that presented with a single large, intra-abdominal tumor nodule within the left ovarian bursa, as well as multiple small nodules on the surface of other organs and tissues. This technique may also be used to refine other tumor models, using the ovarian bursa as an implant site for heterotopic tumor isografts.
Document Type: Research Article
Affiliations: 1: Department of Comparative Medicine, School of Medicine, University of Washington—Seattle, Seattle, Washington 2: Department of Bioengineering and Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington—Seattle, Seattle, Washington 3: Department of Biobehavioral Nursing and Health Systems, University of Washington—Seattle, Seattle, Washington
Publication date: 2004-12-01
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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