Simple Duplex Fecal PCR Assay That Allows Identification of False-Negative Results in Helicobacter sp.-Infected Mice
Abstract:We designed a simple and sensitive duplex polymerase chain reaction (PCR) assay for detection of false-negative results during routine Helicobacter sp. feces analysis. We took advantage of the various Lactobacillus species that form part of the normal intestinal flora of laboratory rodents to improve our PCR diagnostic assays. Using this onestep PCR assay, we were able to rule out false-negative results without the need of adding internal standard molecules. This is an important quality control for PCR diagnostic tests, since the presence of inhibitors in feces can prevent detection of Helicobacter infections using PCR analysis. Use of this Lactobacillus group-specific PCR assay can be extended to other feces tests used in mouse quality-control programs.
Document Type: Research Article
Affiliations: 1: Animalerie Centrale, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France 2: Animalerie Centrale, Unité de Génétique des Mammifères, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France 3: The University of Texas M. D. Anderson Cancer Center, Science-Park Research Division, 1808 Park Road 1C, Smithville, Texas 78957 4: INRA Jouy-en-Josas, Unité d'Ecologie et de Physiologie du Système digestif, Bâtiment 405, 78352 Jouy en Josas Cedex, France
Publication date: October 1, 2004
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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