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Open Access Minimally Invasive Surgery via Laparoscopy for Intra-Abdominal Biopsy in Obese Rhesus Macaques (Macaca mulatta)

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Purpose. To determine the safety and effectiveness of laparoscopy for repeated intra-abdominal biopsy of liver and omental adipose tissue (AT) in obese rhesus monkeys (Macaca mulatta).

Methods. Nine obese rhesus monkeys were studied by use of 18 laparoscopic procedures (two procedures each, approx. six weeks apart). Time-sensitive liver and omental AT specimens were obtained from monkeys under general anesthesia, using a three-port approach with a roticulating endoscopic stapler/divider and a monopolar electrosurgery for hemostasis.

Results. All subjects tolerated the initial and repeat laparoscopic procedures well. Liver specimens weighed a mean ± SEM of 3.8 ± 0.5 g, and omental AT specimens weighed 16.6 ± 0.8 g. Compared with previous studies of conventional laparotomy with liver wedge resection, the monkeys experienced faster postoperative recovery via laparoscopy, with rapid return to normal food intake and activity. Minimal to no adhesions were observed by use of the repeat procedure in all monkeys, with no major complications.

Conclusions. Laparoscopy in obese rhesus monkey (ranging from young to older-aged), with repeated intra-abdominal liver and omental AT biopsy, was an excellent minimally invasive surgical method. In contrast to laparotomy with wedge resection, this approach greatly decreases operative time and stress, provides generous tissue specimens in a time-efficient manner, and facilitates rapid and full recovery of the nonhuman primate.

Document Type: Research Article

Affiliations: 1: Department of Surgery, University of Maryland Medical Center, Baltimore, Baltimore, Maryland 21201 2: Obesity and Diabetes Research Center, Department of Physiology, 10 S. Pine St. MSTF 6-00, School of Medicine, University of Maryland, Baltimore, Baltimore, Maryland 21201

Publication date: 2004-04-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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